Resistance to Spectinomycin Determined by R Factors of Various Compatibility Groups
نویسندگان
چکیده
منابع مشابه
Mechanisms of antibiotic resistance determined by resistance-transfer factors.
Unowsky, Joel (Northwestern University Medical School, Chicago, Ill.), and Martin Rachmeler. Mechanisms of antibiotic resistance determined by resistance-transfer factors. J. Bacteriol. 92:358-365. 1966.-This study was concerned with the mechanism of expression of drug resistance carried by resistance-transfer (R) factors of two types: fi(-) (negative fertility inhibition) and fi(+) (positive f...
متن کاملA spectinomycin resistance determinant from the spectinomycin producer Streptomyces flavopersicus.
The spectinomycin (sp) resistance determinant from Streptomyces flavopersicus was cloned into Streptomyces lividans using the plasmid vector pIJ699. A plasmid, pDGL15, with a 3.65 kb insert from S. flavopersicus conferring resistance to Sp was isolated. DNA sequence analysis of the 3651 1 bp DNA insert revealed four open reading frames (ORFs). The amino acid sequence deduced from one ORF (SpcN)...
متن کاملTwo distinct types of trimethoprim-resistant dihydrofolate reductase specified by R-plasmids of different compatibility groups.
R-Plasmids from a number of trimethoprim-resistant Escherichia coli and Citrobacter sp. were studied after transfer to E. coli K12 hosts. Each was found to specify a dihydrofolate reductase which was resistant to trimethoprim and Methotrexate, and which could be completely separated from the host chromosomal enzyme by gel filtration. Two distinct types of R-plasmid dihydrofolate reductases were...
متن کاملRibosomal resistance to streptomycin and spectinomycin in Neisseria gonorrhoeae.
A cell-free protein synthesizing system was used to study the mechanism of resistance to streptomycin (Str) and spectinomycin (Spc) in laboratory mutants and clinical isolates of Neisseria gonorrhoeae. The 70S ribosomes from sensitive strains were sensitive to the effects of Str and Spc on synthesis directed by several synthetic polynucleotide messengers, whereas 70S ribosomes from resistant st...
متن کاملSeedling tests for resistance to kanamycin and spectinomycin
Methods pPRV111A::neoSTLS2 construction and transplastomic plants pPRV111A::neoSTLS2 was produced by ligating the HindIII fragment containing neoSTLS2 from plasmid pCMneoSTLS2 (ref. 13) into pPRV111A. This transformation vector was introduced into the plastids of Nicotiana tabacum L. Petit Havana (N,N) by biolistic bombardment, and homoplasmic plants were regenerated as described previously. Th...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1972
ISSN: 0022-1287
DOI: 10.1099/00221287-72-2-407